COXSACKIE VIRUS-DERIVED VECTOR SYSTEM FOR GENE TRANSFER - Patent ...
The RNA molecule can be encoded by a vector plasmid and/or can be packaged as a ... d) Ligieren des Amplifikates mit einer DNA-Sequenz für das Fremdgen. ...
http://www.freepatentsonline.com/EP1210439.html[PDF]
Charakterisierung von NUANCE, einem Protein der α-Aktinin Superfamilie
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pGEM-Teasy-Vector System I. Promega ... ligieren. Das GFP-Fusionsprotein enthält die Aminosäuren 6.833-6.879 des C-. terminalen Bereichs von NUANCE, ...
http://www.uni-koeln.de/med-fak/biochemie/dis...[PDF]
POLYMERASEKETTENREAKTION (PCR)
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Klonierungsvektor zu ligieren und das hergestellte Plasmid in Escherichia coli ... Vector Systems are convenient systems for the cloning of PCR products. ...
http://www.vt.tuwien.ac.at/scripts/166014/1_P...[PDF]
Themenliste praktikumsbegleitende Seminare:
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und dann auch ligieren. Trivialerweise also verbinden sich nur Enden, ... digest 1µg vector for vectors <= 3kb, 2µg for vectors larger than that ...<br/>
http://www.biologie.uni-erlangen.de/.../Metho...Problem Solutions for Bacterial Genetics
Vector self-ligate in a circle. Vector ligate to another vector. Insert can ligate to itself, or to another insert. Vector can ligate two copies of insert. ...
http://biology.kenyon.edu/courses/biol114/TUT...ligate 3 DNA fragments with sticky ends into Vector-Molecular Biology
firstly, I cut A, B out of vector, then ligate them, run a gel, cut the right size band(A+B=3.5kb), recover it, then Conform the DNA by BamHI cut. ...
http://www.protocol-online.org/biology-forums...how to ligate random oligos to vector to construct a library ...
Cut this with restriction enzymes at sites in the 5' and 3' ends, and ligate into your vector. You'd want to avoid high temperature denaturing conditions ...
http://www.protocol-online.org/biology-forums...BioTechniques® Molecular Biology Techniques Forums :: View topic ...
Trying to ligate two 500kb pcr fragments into one vector. ... Tried to ligate fragment into vector, get no difference in colony numbers with or without ...
http://molecularbiology.forums.biotechniques....Ligase
ist ein Enzym, das in der Lage ist, die zwei DNA-Doppelstränge - aber auch andere Moleküle - miteinander zu verbinden. Gentechniker brauchen ein solches ...
http://www.aerztezeitung.de/docs/2000/10/06/1...Ligase - Wikipedia, the free encyclopedia
Generally ligase catalyses the following reation:. Ab + C → A–C + b or sometimes ... The common names of ligase enzymes often include the word "ligase", ...
http://en.wikipedia.org/wiki/Ligase
